Skip to main content
. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):165–173. doi: 10.4049/jimmunol.181.1.165

Figure 6. Adoptive transfer of WT or MHC class II-deficient B cells restores chimerism and tolerance.

Figure 6

(A) B cell-deficient μMT and WT B6 mice received T cell-depleted B10.A BMT following 3 Gy TBI/anti-CD154. An additional group of B cell-deficient μMT mice also received 20-23×106 purified B cells from WT B6 mice on Day 0. The incidence of WBC granulocyte chimerism is shown at indicated times. One of two similar experiments is shown (3-7 animals/group/experiment). (B) WBC granulocyte and CD4 chimerism levels (±SEM) for the groups in A. Circles: granulocyte chimerism. Diamonds: CD4 T cell chimerism. Open symbols: WT B6 recipients. Closed symbols with continuous line: μMT recipients. Closed symbols with dashed lines: μMT recipients of WT B6 B cells. (C) Chimeras shown in A and B received donor-type B10.A and 3rd party B10.RIII skin grafts 5 weeks after BMT. Graft survival is shown over time. (D) B cell-deficient μMT and WT B6 mice received T cell-depleted B10.A BMT after 3 Gy TBI/anti-CD154 conditioning. Some μMT mice also received 20-23×106 purified B cells from WT B6 mice or CIITa-/- (MHC class II-deficient) mice on Day 0. The incidence of WBC granulocyte chimerism is shown at indicated times (3-7 animals/group/experiment).