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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):165–173. doi: 10.4049/jimmunol.181.1.165

Figure 7. Requirement for recipient DCs for CD8 T cell tolerance induction.

Figure 7

Lethally irradiated congenic CD45.1 B6 mice were reconstituted with either WT B6 (B6) or DTR-transgenic (DTR tg) B6 BM. Eight weeks later they received fully MHC-mismatched B10.A BMT with 3 Gy TBI/anti-CD154. Some animals received diphtheria toxin to deplete DCs (see Methods), with or without depleting anti-CD8 mAb. (A) One mouse from each group was euthanized on Day 18. The spleen was harvested, digested with collagenase and then stained with CD11c-PE and, anti-CD8β-APC and analyzed by FCM. The percentage of CD11c+ DCs among total lymphocytes is shown. Efficiency of DC depletion was 90-95%. The incidence of B cell chimerism (B) and percent donor B and CD4 cell chimerism (±SEM) (C and D) is shown. One of two experiments is presented (7-8 animals/group/experiment). The repeat experiment included T cell-depletion of the allogeneic BM, with similar results.