FIG. 1.—

Dinoflagellate SL RNAs are 50–60 nt. Polyacrylamide gel electrophoresis of total RNA (A), and blot hybridization of probes for SL RNA using probe DinoSLa/s (B), and 5S rRNA using probe Dino5S (C). Lane 1, Karenia brevis; 2, Polarella glacialis; 3, Karlodinium veneficum; 4, Pfiesteria piscicida; 5, Prorocentrum minimum; and 6, Leishmania tarentolae. Size standards (marked on the left and the right) are L. tarentolae 5.8S rRNA (175 nt), 5S rRNA (110 nt) and tRNA^Gly (72 nt), and the RNA blots (B,C) were aligned with the gel so that the size standards apply for the RNA blots. The single band of K. veneficum SL RNA transcript (marked by an arrow) has been proven to be 56 nt in length by RACE-based cloning and sequencing (Zhang, Hou, et al. 2007).