Table 2.
Oligonucleotides Used in This Study
| Primer Name | Sequence (5′–3′) | Application; Referencea |
| dinoSLg-F | cgagagtatcAGCCATTTTGGCTCAAGb | PCR of genomic SL RNA tandem repeats; Zhang, Hou, et al. (2007) |
| dinoSLg-R | acagaacaAGCCAAAATGGCTACGGb | PCR of genomic SL RNA tandem repeats; Zhang, Hou, et al. (2007) |
| dino5SF1 | GCCATACCGTGTCGAATGC | 5S rRNA forward primer |
| dino5SF2 | CGACCTCCGAAGTTAAGCG | 5S rRNA forward primer |
| dino5SR1 | ACAGCACCCTAAGGWCTTCC | 5S rRNA reverse primer |
| KbrSL1-F1 | AGCCATTTTGGCTCAAGGTACAAG | KbrSL-1 forward primer |
| KbrSL3-F1 | CGTAGCCATTTTGGCTCAAGGC | KbrSL-3 forward primer |
| KbrSL4-F1 | AGCCATTTTGGCTCAAGGTCTAC | KbrSL-4 forward primer |
| KmiSL-F3 | GCTCAAGGTACAAGTTGGGCTG | KbrSL-1 forward primer; Zhang, Hou, et al. (2007) |
| KbrSL3-F2 | AGCCATTTTGGCTCAAGGCACC | KbrSL-3 forward primer |
| KbrSL4-F2 | ATTTTGGCTCAAGGTCTACATCTG | KbrSL-4 forward primer |
| dinoSLa/s | TGTACCTTGAGCCAAAATG | General dinoflagellate SL RNA detection; Zhang, Hou, et al. (2007) |
| dinoSL | NCCGTAGCCATTTTGGCTCAAG | PCR amplifying dinoflagellate full-length cDNAs as well as SL RNA genomic DNA |
| dino5Sa/s | GGACTTCCCGGGCGGTC | General 5S rRNA detection |
| KbrSL-4a/s | AGCCCAGATGTAGACCT | Karenia brevis SL RNA type SL-4 detection |
a
Oligonucleotides from this study show no reference.
b
Lowercase letters represent random nucleotides.