FIG. 2.—

Examples of hybridization experiments on gibbon and human with α-PCR products. FISH experiments using probe α27/α30-NLE PCR product on NLE (A) and human metaphases (B). (C) Hybridization signals using α27/α30-HSA amplification product on human chromosomes. No signals were detected on chromosome Y indicated by an arrow. (D) Cohybridization using the clone pGamma_36 (green) and a telomeric probe (telomere PNA FISH, kit Cy3, DAKO) (red) showing colocalization of telomeric and centromeric sequences in gibbon chromosomes. (E) The figure shows the colocalization of NLE α satellite clone pGAMMA_41 with human and gibbon BAC clones that cover NLE EBs reported by Roberto et al. (2007) (see text and supplementary table S9, Supplementary Material online, for details).