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. 2009 Sep 7;4(9):e6936. doi: 10.1371/journal.pone.0006936

Figure 2. Ectopic ESCO2 expression during the cell cycle.

Figure 2

VU1199-F SV40 cells stably expressing V5-ESCO2 were synchronized by a double aphidicolin block and mitotic shake-off and samples were analyzed for (A) ESCO2 expression on Western blot and (B) DNA content by flow cytometry. (C) Cell cycle distribution related to GFP-ESCO2 expression was measured by flow cytometry in VU1199-F SV40 cells stably expressing wild type or mutant GFP-ESCO2. Cells were fixed in 70% ethanol and DNA was stained with ToPro3. Asterisk indicates a protein detected by the ESCO2 antibody which is supposed to represent a degradation product of ESCO2.