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. 2009 Sep 7;4(9):e6936. doi: 10.1371/journal.pone.0006936

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van der Lelij et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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VU1199-F SV40 fibroblasts, VU1199-F corrected with V5-ESCO2 and wild type fibroblasts LN9SV were left untreated or exposed to MMC, camptothecin (CPT) or hydroxyurea (HU) for 24 h and whole cell extracts were obtained to investigate the phosphorylation of Chk-1. Vinculin and Chk-1 served as loading controls.