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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Jan 15;29(4):480–487. doi: 10.1161/ATVBAHA.108.181925

Figure 3.

Figure 3

Characterization of primary-cultured aortic smooth muscle cells from Nox1y/- and TgSMCnox1 mice. A. PCR was conducted with primer pairs that can distinguish cDNA of Nox1y/- cells from that of WT or TgSMCnox1 cells. PCR products were detected on agarose gel electrophoresis. A representative gel image is presented. B. Nox4 protein expression was measured by Western blotting. CDK4 was measured as a loading control. Representative images are shown in upper panels. Values are means±SE from 3 independent experiments. C. Serum-starved VSMCs were treated with 25 ng/ml PDGF for 4 h, which corresponds to maximal activation of NADPH oxidase. Membrane fractions were prepared, and NADPH-dependent, SOD-inhibitable superoxide generation was measured with ESR using CPH. Values shown are means±SE from 5 independent experiments. *Significantly different from corresponding control (p < 0.05).