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Neurons were incubated with 30 min to 4.5 h 200 nM thrombin, total RNA extracted, reverse transcribed and amplified using primers specific for cdk2, and the housekeeping gene β-actin (n = 3). Upper panel: cdk2 300 bp amplicon, lower panel: β-actin 392 bp amplicon. ***p < 0.001 vs. control
