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Cortical neurons were incubated 30 min to 4.5 h with 200 nM thrombin, lysed and protein separated by SDS-PAGE, transferred to a PVDF membrane and immunoblotted for cdk2 (25–30 μg/lane). Lysate from brain endothelial cells was used as a positive control. GAPDH was measured to confirm loading equivalency among lanes (n = 3). ***p < 0.001 vs. control
