Table 1.
Primers used for the polymerase chain reaction (PCR)
| Gene | GI number | Primers | Amplicon size |
|---|---|---|---|
| β actin | 42475926 | Fwd 5'TGTCACCAACTGGGACGATA 3' Rev 5'TCTCAGCTGTGGTGGTGAAG 3' |
392 bp |
| Cyclin C | 109476214 | Fwd 5' CATGTGTGTTTCTGGCATCC 3' Rev 5' TCCGTCCTGTAGGTGTCATTC 3' |
295 bp |
| Cdk2 | 41054835 | Fwd 5' CATTCCTCTTCCCCTCATCA 3' Fwd 5' GTCACCATTTCGGCAAAGAT 3' |
300 bp |
| Cdk1 | 9506474 | Fwd 5' TTGAAAGCGAGGAAGAAGGA 3' Fwd 5' CCGAAATCTGCCAGTTTGAT 3' |
334 bp |
| p27 | 50054215 | Fwd 5' CAGAATCATAAGCCCCTGGA 3' Fwd 5' TCTGTTCTGTTGGCCCTTTT 3' |
320bp |