Skip to main content
. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Cancer Lett. 2006 May 4;247(1):130–136. doi: 10.1016/j.canlet.2006.03.029

Fig. 2.

Fig. 2

Validation of transfected HEK293F cells. (a)–(c) Flow cytometry analysis: HEK293F cells transfected with (a) MSLN1 or (b) mesothelin constructs were incubated with 10 μg/ml of 4H3 mAb (solid lines); (c) HEK293F cells transfected with MPF construct were incubated with 10 μg/ml of anti-c-myc tag mAb (solid lines). As negative controls, wild type (dotted line) or transfected cells (shade areas) were incubated with the secondary antibody only. (d)–(e) Western blot: (d) Meso-Ig and (e) MPF-Ig chimeric proteins were detected with an HRP-anti-hIg mAb.