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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Clin Cancer Res. 2006 Apr 1;12(7 Pt 1):2117–2124. doi: 10.1158/1078-0432.CCR-05-2007

Figure 1. Determination of optimal coupling concentrations with purified CA125 antigen for five pairs of anti-CA125 antibodies.

Figure 1

Five coupling concentrations of capture antibody (0.2, 1, 5, 10 and 20 μg/ml) were incubated with serial dilutions of CA125 antigen in the assay diluent. The captured antigen was detected by 1 μg/ml of biotinylated detection antibody. A: RDI antibodies, X306 capture, X52 detection; B: FDI antibodies, M11 capture, OC-125 detection; C–D: FIII antibodies, C: M002201 capture, M002203 detection; D, orientation 1: M8072320 capture, M8072321 detection; orientation 2: M8072321 capture, M8072320 detection.