Table 2. Reproducibility and validity of the bead-based assays.
Reproducibility of case and control measurements for the CA125 RDI, CA125 FIII and HE4 bead-based assays was assessed by Pearson correlation (r) between two replicate experiments, calculated on the log scale.
Validity of the CA125 RDI and FIII bead-based assays was assessed by Pearson correlations with the research standard CA125II RIA (CA125 RDI/RIA and CA125 FIII/RIA respectively), calculated on the log scale.
| Reproducibility | Validity | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| CA125 RDI assay | CA125 FIII assay | HE4 assay | CA125 RDI/RIA | CA125 FIII/RIA | |||||
| N | r | N | R | N | r | N | r | r | |
| Overall | 197 | 0.99 | 195 | 0.95 | 194 | 0.95 | 204 | 0.95 | 0.91 |
|
| |||||||||
| Cases | 64 | 1.00 | 64 | 0.93 | 62 | 0.94 | 66 | 0.93 | 0.88 |
|
| |||||||||
| Non-cases (total) | 133 | 0.94 | 131 | 0.89 | 132 | 0.89 | 138 | 0.85 | 0.80 |
| • Surgical | 69 | 0.98 | 68 | 0.97 | 69 | 0.90 | 72 | 0.91 | 0.81 |
| • Screening | 64 | 0.83 | 63 | 0.64 | 63 | 0.86 | 66 | 0.64 | 0.73 |