TABLE 3.

List of Proteins Differentially Affected by 1,2-DAB (100% Confidence Score for Protein ID by MALDI-TOF/MS-MS)

Spot number	Protein name	Symbols	Accession number	Molecular weight	Isoelectric point	Peptides matched	Function	Fold changea
1	Fatty acid synthase	Fasn	204099	272,493.9	5.96	27	Metabolism of lipids	−1.65
2	ATP citrate lyase	Acly	8392839	120,558.7	6.96	23	Energy metabolism	−1.57
3	Endoplasmin		51858886	74,161.6	5.02	26	Heat shock protein	−1.44
4	Formyltetrahydrofolate dehydrogenase	Fthfd	57921067	98,811.5	5.64	34	Detoxification	−2.52
5	Gelsolin	Gsn	51854227	86,014.1	5.76	16	Protein binding/structural protein	−2.42
6	Hydroxyacyl-coenzyme A dehydrogenase/3-ketoacyl-coenzyme A thiolase/enoyl-coenzyme A hydratase (TFP), alpha subunit	Hadha	60688124	82612.5	9.16	22	Energy metabolism	−1.69
7	Dihydrolipoamide acetyltransferase	Odp2	34863356	67,123.4	8.76	12	Energy metabolism	−1.63
8	Pyruvate kinase muscle	Pkm2	56929	57,780.9	6.63	18	Energy metabolism	+2.02
9	PDI related 3	Pdi	38382858	56,587.7	5.88	20	Protein folding	−2.86
10	3-Hydroxy-3-methylglutaryl-coenzyme A synthase 1 (Hmgcs1)		8393538	57,397.4	5.58	16	Energy metabolism	−2.87
11	GDP dissociation inhibitor 2	Gdi2	40254781	50,504.7	5.93	18	Catalytic activity/vesicle trafficking	−1.19
12	Aldolase A	Aldoa	6978487	39,327.3	8.31	14	Catalytic activity	+1.85
13	NADH dehydrogenase (ubiquinone) 1 alpha	Ndufa10	46391108	31,680.8	5.44	9	Energy metabolism	+2.39
14	NADH dehydrogenase (ubiquinone) 1 alpha	Ndufa10	46391108	31,680.8	5.44	12	Energy metabolism	−1.83
15	Glyceraldehyde-3-phosphate dehydrogenase	Gpd	56611127	35,771.2	8.14	6	Energy metabolism	+1.38
16	Voltage-dependent anion channel-like protein	—	540011	31,699.6	7.44	7	Unknown	−2.1
17	Glyceraldehyde-3-phosphate dehydrogenase	Gpd	8393418	35,805.2	8.14	7	Energy metabolism	+6.8
18	Similar to biliverdin reductase B (flavin reductase)	Blvrd	34855391	22,080.3	6.29	9	NADPH diaphorase activity	−2.9
19	Glutathione-S transferase Yb-1 subunit (EC 2.5.1.18)	Gstm1	204503	25,899	7.63	13	Detoxification	−5.24
20	Glutathione-S transferase Yb-1 subunit (EC 2.5.1.18)	Gstm1	204503	25,899	7.63	17	Detoxification	+2.49
21	Quinoid dihydropteridine reductase	Dhpr	11693160	25,535.9	7.67	9	NADPH dihydropteridine reductase activity	+1.89
22	NF, light polypeptide	Nefl	13929098	61,298	4.63	17	Protein binding/structural protein	−1.61

^aMaximum fold change values observed after cross-gel analysis of proteomic expression. Negative or positive values indicate low or high expression level, respectively, of the indicated protein in samples treated with neurotoxic 1,2-DAB. Dual protein expression of certain proteins (e.g., NADH) may possibly be explained by a concomitant expression of isoforms, or protein subunits, or post-translationally modified proteins. Protein no. 16 has been removed from the nrNCBI databases.