FIG. 5.

Oligonucleotide extension analysis of toxin-induced cleavage of 28S rRNA S/R loop in RAW 264.7 cells. Cells were incubated with DON, T-2, and ricin RAW cells were treated with DON (1000 ng/ml), T-2 (80 ng/ml), and ricin (160 ng/ml) for 4 h. Reverse transcription reaction was performed on total RNA using FAM-labeled primer 1–specific for mouse 28S rRNA from 4300 to 4281 (depicted in Fig. 6). Resultant cDNA extension fragments were sized and quantified. Results are representative of two separate experiments.