FIG. 2.

Submitochondrial compartmentation of Trx2. Experiments were performed in which increasing concentrations of digitonin were added to isolated mitochondria. Mitochondria were pelleted by centrifugation to allow detection of released proteins in the supernatant. Western blotting was used to determine whether Trx2 was present in the intermembrane space or in the matrix space. Trx2 was released (top and middle panels) only at high concentrations of digitonin, whereas the intermembrane space marker AK (bottom panel) was released at low concentration of digitonin. Data from a single experiment which is representative of data from three separate mitochondrial preparations.