FIG. 4.

Trx2 protection against oxidant-induced MPT. To determine whether Trx2 protected against oxidant-induced toxicity, liver mitochondria from Tg and WT mice were assayed for the MPT in the presence of 20μM Ca²⁺ and 3mM inorganic phosphate, with additions of 0, 12.5, 25, or 50μM tBH. Data are presented as the percentage of the maximal MPT response obtained under the conditions indicated at 20 min. Results are means ± SEM from experiments on four pairs of Tg and WT littermates. Data for respective Tg and WT pairs were significantly different at all tBH concentrations. Data were analyzed by ANOVA with post hoc analysis by Student-Newman-Keuls test. *Significant at p < 0.05.