FIG. 1.

General study design. Human TK6 cells were treated for 4 h with either cisplatin, a direct genotoxin, etoposide, a topoisomerase inhibitor, taxol, an aneugen, or sodium chloride, a cytotoxic clastogen. Then the compound was washed out and cells were further cultivated up to 24 h. Cell samples were taken after 2 (optional), 4, 7 (optional), and 24 h after treatment start. The cell number relative to untreated controls was determined as cytotoxicity parameter. RNA was isolated from at least the 4- and 24-h sample and processed for expression analysis of 47 genes with Taqman technology (Taqman card system or Taqman Assays on Demand).