Skip to main content
. 2009 May 13;29(19):6320–6335. doi: 10.1523/JNEUROSCI.4630-08.2009

Figure 9.

Figure 9.

Synaptic AMPA-R trafficking regulates synaptic integration. A, Simultaneous recordings, made under transmitted light illumination (top), from pairs of nearby recombinant protein expressing layer 4 neurons, identified by GFP (middle) and RFP (bottom) fluorescence. B, Left, Asynchronous quantal EPSCs from intracortical (IC) synapses of GluR4ct–RFP- and GluR1ct–GFP-expressing neurons and from thalamocortical (TC) synapses of GluR4ct–RFP- and GluR2ct–GFP-expressing neurons. Top right, Rise time of asynchronous quantal EPSCs from intracortical synapses of GluR4ct–RFP- and GluR1ct–GFP-expressing neurons (GluR4ct–RFP, 0.88 ± 0.04 ms; GluR1ct–GFP, 0.62 ± 0.04 ms; n = 11; p < 0.005) and from thalamocortical synapses of GluR4ct–RFP- and GluR2ct–GFP-expressing neurons (GluR4ct–RFP, 0.89 ± 0.04 ms; GluR2ct–GFP, 0.57 ± 0.02 ms; n = 10; p < 0.01). Bottom right, Decay time constant of asynchronous quantal EPSCs from intracortical synapses of GluR4ct–RFP- and GluR1ct–GFP-expressing neurons (τ; GluR4ct–RFP, 3.62 ± 0.23 ms; GluR1ct–GFP, 2.60 ± 0.15 ms; n = 11; p < 0.005) and from thalamocortical synapses of GluR4ct–RFP- and GluR2ct–GFP-expressing neurons (τ; GluR4ct–RFP, 3.68 ± 0.25 ms; GluR2ct–GFP, 1.98 ± 0.12 ms; n = 10; p < 0.01). C, Top, Ten consecutive superimposed intracortical EPSPs in a pair of nearby GluR4ct–RFP-and GluR1ct–GFP-expressing neurons. Bottom, Ten consecutive superimposed thalamocortical EPSPs in a pair of nearby GluR4ct-RFP and GluR2ct-GFP-expressing neurons. Overlay includes three representative evoked responses from each cell pair. D, Top, Percentage of second EPSPs that trigger action potential in GluR4ct–RFP- and GluR1ct–GFP-expressing neurons (GluR4ct–RFP, 46.0 ± 6.6%; GluR1ct–GFP, 3.2 ± 1.3%; n = 10; p < 0.01). Bottom, Range of action potential jitter (GluR4ct–RFP, 10.1 ± 3.8 ms; GluR2ct–GFP, 2.1 ± 0.5 ms; n = 8; p < 0.05) and EPSP–action potential time delay (GluR4ct–RFP, 7.5 ± 0.4 ms; GluR2ct–GFP, 6.2 ± 0.5 ms; n = 8; p < 0.05) in GluR4ct–RFP- and GluR2ct–GFP-expressing neurons. *p < 0.05 (Wilcoxon's test).