Skip to main content
. 2009 Sep 9;4(9):e6931. doi: 10.1371/journal.pone.0006931

Figure 3. Decreased MSI following AP-2α downregulation in HNSCC cell lines SCC22B and SCC17as.

Figure 3

a) 9 MSI markers were used with DNA from SCC22B and SCC17as cells with and without AP-2α downregulation, and the products were analyzed on an 8% PAGE gel. b) ChIP analysis using an AP-2α antibody in SCC22B cells with and without AP-2α downregulation. ChIP eluate was then utilized for quantitative PCR using MLH1 promoter primers. c) Bisulfite sequencing analysis of 3 additional DNA repair genes in WT SCC22B cells. Because no methylation was observed in the WT cells, it was unnecessary to analyze methylation in AP-2α downregulated cells. Solid circles represent methylated CpGs; whereas, open circles represent unmethylated CpG sites.