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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Eur J Immunol. 2008 Dec;38(12):3411–3425. doi: 10.1002/eji.200838432

Figure 3. Microbiota in RF mice plays a role in the formation of MZ B cells.

Figure 3

RF neonates were treated beginning at post-natal day 0 (P0) with lumenal bacteria from SPF mice (see Methods). The mice were examined for MZ B cell development at age of about 8 weeks old. (A) Phenotypes of splenic CD19+ B-cells; % MZ B cells in CD19+ gate are indicated. (B) Tabulation of % MZ B cells; each symbol represents data from an individual mouse. SPF: specific pathogen free mice; RF: restricted microbiota mice; RF+BactSPF P0: RF pups exposed to SPF cecal bacteria at post-natal day 0 (P0). RF+BactSPF P0+Vanco: Offspring derived from RF parental mice that were treated with vancomycin under SPF condition. These pups were exposed to SPF environment during and immediately after birth. P values by student t test for comparisons of SPF mice to RF and bacteria-treated RF mice. Data was obtained from 3 or more independent experiments.