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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Eur J Immunol. 2008 Dec;38(12):3411–3425. doi: 10.1002/eji.200838432

Figure 4. Profound deficiency of MZ B cells in RF mice but not in germ-free mice.

Figure 4

(A) Splenic lymphocytes were isolated from age and gender-matched SPF, germ-free (GF) and RF mice, and stained to delineate MZ B cells (CD19, CD21, IgM and IgD). The percentages of cell populations with MZ B cell phenotypes are indicated. Data represents at least three independent experiments, and two different strains (C57/BL6 and Swiss Webster) of germ-free mice. (B–D) Tabulation of data from individual age-matched mice (8–10 weeks) reared in SPF, GF, or RF conditions: (B) Percentage of CD19+ B cells; (C) % MZ B cells; (D) total MZ B cells per spleen. P values were calculated using student’s t test comparison of GF or RF to the control SPF group.