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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Methods Mol Biol. 2008;419:215–230. doi: 10.1007/978-1-59745-033-1_15

Fig. 3.

Fig. 3

Translation of firefly luciferase mRNA with increasing length poly(A) in RNA-transfected LM(tk−) cells. A. Triplicate cultures of LM(tk−) cells were transfected with the indicated firefly luciferase transcripts with varying length poly(A) tails together with a Renilla luciferase RNA control as in Fig. 2. Firefly luciferase activity was normalized to that of co-transfected Renilla luciferase and the results are plotted as in Fig. 2. B. RNA extracted from the transfected cells was analyzed by Northern blot as in Fig. 2 using a mixed probe for firefly (Fluc) and Renilla (Rluc) luciferase mRNA.