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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Cancer Res. 2008 May 1;68(9):3169–3177. doi: 10.1158/0008-5472.CAN-07-6636

Figure 3. Michaelis-Menten analysis of ligase inhibitors: Effect of ligase inhibitors on DNA-protein complex formation by hLigI.

Figure 3

A hLig1 (0.05 pmol) was incubated in the absence (○) and presence of L189 (left panel), L67 (middle panel) and L82 (right panel) at 25 (△) and 50 μM (□) with increasing amounts of a linear nicked DNA substrate. Lineweaver-Burk double reciprocal plots of initial reaction velocity (1/V) versus substrate concentration (1/S) are shown. B. A labeled linear substrate with a single non-ligatable nick (1 pmol) was incubated with; lane 1, no addition; lanes 2 and 3, 0.25 pmol hLigI; lanes 4 and 5, 0.5 pmol hLigI; lanes 6 and 7, 1 pmol hLigI in the absence (−) or presence (+) of 100 μM L189. C. A labeled linear substrate with a single non-ligatable nick (1 pmol) and hLigI (3 pmol) were incubated with either no additon (lane 2) or L82 at; 100 μM (lane 3); 60 μM (lane 4); 50 μM (lane 5); 30 μM (lane 6); 20 μM (lane 7); 10μM (lane 8). Lane 1, 1 pmol of DNA substrate alone. The positions of the labeled DNA substrate (DNA) and DNA-protein complexes (Lig-DNA) are indicated.