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Ubiquitin mRNA is increased in cardiac muscle of diabetic mice. Ubiquitin mRNA was measured by Northern blot analysis of cardiac muscle of control (Ctrl), diabetic (STZ), or insulin-treated diabetic (STZ+Ins) mice. The bar graph of the band densities shows the quantity of ubiquitin mRNA expressed as the percentage of paired control after normalization with the density of the GAPDH band. Date are reported as the means ± se (n = 6 per group). *, P < 0.001 vs. control.
