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. 2007 Oct 25;149(1):208–218. doi: 10.1210/en.2007-1155

Figure 2.

Figure 2

E2 promotes hNPC proliferation in a dose- and time-dependent manner. hNPCs were incubated in a starvation condition (absence of GH and B27 supplement) for 4–6 h, followed by exposure to different concentrations of E2 in the starvation medium for indicated time periods. hNPCs were pulse loaded with BrdU for 2 h, and BrdU incorporation was subsequently measured by chemiluminescence BrdU ELISA. Data were derived from at least three independent experiments conducted with eight replicates. Results were plotted as percentage of vehicle control (mean ± sem). *, P < 0.05. **, P < 0.01. A, E2 induced a dose-dependent increase in BrdU incorporation. The minimally effective concentration was 1 nm E2 (18 ± 10% increase), with the maximal proliferative effect achieved at 100 nm E2 (27 ± 3% increase). B, E2-induced hNPC BrdU incorporation was time dependent. Significant BrdU incorporation was evident at 3 h (17 ± 4%), reached maximum at 5 h (35 ± 8% vs. vehicle control), at which time BrdU incorporation plateaued and was sustained for 24 h. The positive control, complete medium with GH and B27 supplement, induced a 29 ± 2% increase in BrdU incorporation vs. control.