Figure 5.

E₂-induced hNPC proliferation is mediated by ERβ. Cultures of hNPCs were incubated in the presence or absence of E₂, PPT (0.5 nm), or DPN (0.3 nm) for 24 h. Proliferation was determined by BrdU ELISA (A) and total cell number (B). E₂ and ERβ-selective agonist, DPN, significantly increased BrdU incorporation and total cell number, whereas the ERα-specific ligand, PPT, did not. Data are presented as mean ± sem from three independent experiments. *, P < 0.05 vs. control or as indicated.