Comparison of Treg expression, multiorgan inflammation, and disease manifestation among OT-II, Sf, and OT-II Sf mice. Male B6, male OT-II, Sf, and OT-II Sf mice were examined for CD4+Foxp3+ Treg (A; n = 3, 3–4 wk old), body weight gain (B; n = 6), survival rate (C; n = 6), and multiorgan inflammation (D; n = 5). As compared with Sf mice, OT-II Sf mice (5–6 wk old) had increased body weight gain and prolonged life span that resulted in inflammation in multiple organs examined (D). To determine whether polyclonal-activated T cells induced multiorgan inflammation, adult B6 mice (n = 3, 9 wk old) were treated with anti-CD3 mAb as described in Materials and Methods and various organs were examined (D).