Table I.
Name | Sequence (5′→3′)a |
---|---|
dual_0 | TCG CCA GGG CTT TCC (AF-T)AG TCA CGA CCG TAA AAC GAC GGC CA-Q |
DUAL | AF-TAG GAG GGC TTT CCT CCT CAC GAC CGT CAA ACG ACG GTC A-Q |
UD | TAG GAG GGC TTT CCT CCT CAC GAC CGT CAA ACG ACG GTC A |
*U | AF-TAG GAG GGC TTT CCT CCT CAC GAC C |
D° | GTC AAA CGA CGG TCA-Q |
T | CTG ACC GTC GTT TGA CGG TCG TGA GGA GGA AAG CCC TCC TA |
UT | CTG ACC GTC GTT TGA CGG TCG |
DT | TGA GGA GGA AAG CCC TCC TA |
“AF” and “Q” indicate the positions of the Alexa Fluor 488 and Black Hole Quencher-1 moieties, respectively, linked to the 5′- or 3′-termini of applicable DNA substrates. For probe dual_0, the Alexa Fluor 488 dye was conjugated to an internal T residue.