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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6199–6207. doi: 10.1158/0008-5472.CAN-07-6514

Figure 2. Insertion of the PHD fingers allows the recruitment of Cyp33 & HDAC1.

Figure 2

All fusion protein constructs in the pCMV2 vector were cotransfected with HA tagged Cyp33 construct into 293 cells. The fusion proteins were immunoprecipitated with anti-FLAG antibody and any bound HA-Cyp33 or endogenous HDAC1 was detected by western blot analysis using anti-HA antibody and anti-HDAC1 antibody respectively. 1% (v/v) whole cell lysate for each sample was used as input. The MLL-AT-hook construct was the negative control. MLL-ENL and MLL-ENL with the ePHDf3 or with PHDf1-3 were tested in (A) and MLL-ENL without the RD2 domain and its ePHDf3 insertion mutant were examined in (B). The data shown here is representative of the results in three independent experiments.