Figure 1b:

(a–d) Immunofluorescence and enzyme-linked immunosorbent assay in cells infected with Ad-HA-SSTR2. Immunofluorescence targeting the hemagglutinin A domain demonstrates cell membrane localization of the fusion protein in (a) L3.6pl and (c) MDA-MB-435 cells after infection with Ad-HA-SSTR2. Cells infected with Ad-GFP show diffuse fluorescence in the cytoplasm in (b) L3.6pl and (d) MDA-MB-435 cells. (e) Graph shows enzyme-linked immunosorbent assay targeting the hemagglutinin A domain before and after infection with Ad-HA-SSTR2 or Ad-GFP. Expression was seen in both cell types infected with Ad-HA-SSTR2. * = P < .001, n = 3, ttest comparing Ad-HA-SSTR2 and Ad-GFP by cell line. mU = milliunits. Error bars = standard deviation.