Table 2.
Calculated and observed molecular weights of full-length and C-terminally truncation Ltn proteins.
| Protein | Expected MWa (Da) |
Observed MWb (Da) |
|---|---|---|
| Ltn(1–93) | 10179 | 10175 |
| Ltn(1–53) | 5794 | 5797 |
| Ltn(1–68) | 7565 | 7595 |
| Ltn(1–72) | 8051 | 8079 |
| Ltn(1–78) | 8718 | 8718 |
| Ltn(1–85) | 9421 | 9418 |
a
Expected molecular weights were calculated from primary sequence using the ProtParam tool on the ExPASy proteomics server (http://ca.expasy.org).
b
Observed molecular weights were determined from MALDI-MS analysis of purified proteins (Figure 6A).