IL-2-dependent regulation of CD103 is specific to CD4+Foxp3- T cells. (A) CD103 expression is not inhibited in the Treg of Il2-/- mice. Lymphocytes were obtained from LN, lungs, liver, skin and colon from B6 and Il2-/- mice (N=4) and stained for CD103, Foxp3 and CD4. The numbers represent mean±S.D. from gated CD4+ T cells. The intensity of staining may be reduced slightly by protease treatment for lymphocyte preparations. Please note the CD103 expression was high on Treg and non-Treg isolated from colon. (B) CD103 expression is down-regulated in Il2-/-, Sf, and Sf.Il2-/- mice as compared with B6 control. LN cells from these mice were stained for CD8 and CD103 as described in Materials and Methods. (C) No significant changes in CD103 expression on CD11c+ DC from Il2-/-, Sf. and Sf.Il2-/- as compared with B6 control. LN cells from these mice were stained for CD11c and CD103 as described in Materials and Methods. Representative data from at least 3 experiments are presented for B and C.