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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Clin Cancer Res. 2009 Jun 9;15(12):3979–3989. doi: 10.1158/1078-0432.CCR-08-1899

Figure 1. Validation of RNA amplification and laser capture.

Figure 1

(A) Genes with no greater than 1.5-fold variation between amplified and unamplified RNA; 88.7% of genes met this criteria (left); genes with greater than 2-fold variation between amplified and unamplified RNAs (right), which constitute only 3% of the total.

(B) Quantitative RT-PCR of PSA expression in laser captured normal and tumor stroma and prostate cancer tissue. PSA content of normal and tumor stroma was not significantly different and both were 1600-fold less than undissected prostate cancer tissue. Mean +/− of triplicate determinations is shown. PSA copy number was normalized to HPRT copy number relative to the Universal Reference RNA.

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