Table 2.
Differential regulated proteins in H44/76Δhfq
Gene ID* | Name† | Location‡ | Reg−§ | Functions¶ | Functional class∥ | MW** | pI†† | Figure‡‡ |
---|---|---|---|---|---|---|---|---|
NMB0018 | PilE | CE | − | Type IV pilus assembly protein PilE | Surface structure | 18 072 | 9.6176 | 4c |
NMB0138 | FusA | W | + | Elongation factor G | Protein translation and modification | 77 244 | 4.8184 | 4a |
NMB0142 | RplC | W | + | 50S ribosomal protein L3 | Ribosomal protein synthesis and modification | 22 678 | 10.7606 | 4a |
NMB0154 | RplE | W | − | 50S ribosomal protein L5 | Ribosomal protein synthesis and modification | 20 322 | 10.0609 | 4a |
NMB0341 | TspA§§ | C | + | T-cell-stimulating protein A | Unknown | 92 488 | 4.0561 | NS |
NMB0430 | PrpB | W, C | + | Putative carboxyphosphonoenol pyruvate phosphonomutase | Carbohydrate metabolism | 31 714 | 5.0352 | 4a |
NMB0431 | PrpC | C | + | Methylcitrate synthase | Carbohydrate metabolism | 42 818 | 7.1147 | NS |
NMB0546 | AdhP | W | + | Alcohol dehydrogenase (propanol preferring) | Fermentation | 36 547 | 5.8432 | 4b |
NMB0554¶¶ | DnaK | W | + | Molecular chaperone | Environmental information processing | 68 791 | 4.5862 | 4a |
NMB0589 | RplS | CE | + | 50S ribosomal protein L19 | Ribosomal protein synthesis and modification | 13 767 | 11.0643 | NS |
NMB0634¶¶ | FbpA | C | + | Iron(III) ABC transporter/major ferric iron-binding protein | Transport/binding proteins | 35 827 | 10.1574 | NS |
NMB0791¶¶ | PpiB | W | + | Peptidyl-prolyl cis–trans isomerase B (cyclophilin B) | Protein translation and modification | 18 852 | 4.8602 | 4b |
NMB0823 | Adk | W | + | Adenylate kinase | Purine ribonucleotide biosynthesis | 23 190 | 4.7879 | 4b |
NMB0884¶¶ | SodB | W, C | + | Superoxide dismutase, Fe–Mn family | Detoxification | 21 892 | 6.1483 | 4b |
NMB0920 | icd | W, W, SP | + | Isocitrate dehydrogenase | Tricarboxylic acid cycle | 80 163 | 5.6646 | 4a, 4b |
NMB0946 | – | W, OM | + | Peroxiredoxin 2 family protein | Unknown | 26 912 | 4.5382 | 4b |
NMB0954 | GltA | W, C | + | Type II citrate synthase | Tricarboxylic acid cycle | 48 121 | 6.7886 | 4a |
NMB1320¶¶ | RplI | W | + | 50S ribosomal protein L9 | Ribosomal protein synthesis and modification | 15 747 | 7.5349 | 4b |
NMB1388 | Pgi-2 | C | + | Glucose-6-phosphate isomerase | Glycolysis | 62 084 | 6.5126 | NS |
NMB1398 | SodC | W | + | Cu–Zn superoxide dismutase | Detoxification, periplasmic protein | 19 520 | 6.6244 | 4b |
NMB1572¶¶ | AcnB | W, IM | + | Aconitate hydratase | Tricarboxylic acid cycle | 92 715 | 5.2810 | 4a |
NMB1584 | – | W, C, C | + | 3-Hydroxyacid dehydrogenase | Amino acid metabolism | 30 378 | 5.1348 | 4b |
NMB1710 | GdhA | W | − | Glutamate dehydrogenase | Amino acid biosynthesis | 48 490 | 6.1895 | 4b |
NMB1796¶¶ | – | W, C | + | Putative oxidoreductase | Metabolism | 20 686 | 5.8148 | 4b |
NMB1934 | AtpD | IM | − | ATP synthase F1, β chain | ATP-proton motive force | 50 391 | 4.7746 | NS |
NMB1936 | AtpA | IM | − | ATP synthase F1, α chain | ATP-proton motive force | 55 291 | 5.2754 | NS |
NMB2091 | – | CE | + | Putative hemolysin | Membranes, lipoproteins and porins | 21 745 | 10.2186 | 4c |
NMB2129 | ArgG | IM | + | Argininosuccinate synthase | Amino acid biosynthesis | 49 664 | 4.9527 | NS |
Gene identification according to Tettelin et al. (2000).
Protein name according to Tettelin et al. (2000).
Fraction in which the protein was identified: W, whole cell lysate; C, cytoplasm; CE, cell envelop; IM, innermembrane; OM, outermembrane; SP, secreted protein fraction.
Up- or downregulation in hfq knockout.
Protein function according to KEGG (http://www.genome.jp/kegg).
Functional classification according to Parkhill et al. (2000).
Protein molecular weight according to JCVI-CMR (http://cmr.jcvi.org/cgi-bin/CMR/shared/Menu.cgi?menu=genome).
Isoelectric point according to JCVI-CMR (http://cmr.jcvi.org/cgi-bin/CMR/shared/Menu.cgi?menu=genome).
Figure in which spot and/or band is identified. NS, not shown.
Protein name according to Oldfield et al. (2007).
Iron-responsive gene (Grifantini et al., 2003; Delany et al., 2004; Basler et al., 2006).