Table 1. Physiological effects of dsRNA-mediated silencing of α-glucosidase.
Accessiona | Putative Function b | E-value | Organism Similarity | Identities (%) |
FJ236283 | Alpha-glucosidase | 3e-145 | Rhodnius prolixus | 100 |
XP_001851486 | Alpha-glucosidase | 8e-102 | Culex quinquefasciatus | 70 |
XP_001869889 | Alpha-amylase | 4e-93 | Culex quinquefasciatus | 64 |
XP_001847532 | Alpha-glucosidase | 5e-90 | Culex quinquefasciatus | 64 |
XP_001656785 | Alpha-amylase | 7e-101 | Aedes aegypti | 69 |
CAA60858 | Maltose like protein Agm2 | 4e-86 | Anopheles gambie | 59 |
AAB82328 | Maltase 2 | 1e-71 | Drosophila virilis | 52 |
PDBc ZEO_A | Alpha-glucosidase | 6e-47 | Geobacillus sp. | 27 |
PDB 1UOK_A | Oligo-1,6-Glucosidase | 1e-25 | Bacillus cereus | 27 |
CAA87020 | Alpha-glucosidase | 2e-22 | Saccharomyces cerevisiae | 24 |
The insects were injected with 2 µL of 100 mM PBS pH 7.4 or dsLacZ (controls) and dsα-glu (2 or 10 µg/female); mortality and oviposition were monitored 4 days after feeding. In all panels, results are means ±SEM (n = 70). The insects injected with 10 µg dsα-glu, analyzed 4 days feeding, were significantly different from control insects injected with both PBS and dsLacZ and also analyzed 4 days after feeding *(P<0.05).
Access number to GenBank database.
Function expected according the Blastx sequence results similarity in GenBank database.
Protein Data Bank.