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MCP-1, sICAM-1, and TNF-a levels in the serum of apoE-/- mice were analyzed by ELISA. IL-1β (a), TNF-a (b), sICAM-1 (c), and MCP-1 (d) levels markedly increased in the serum of apoE mice following different weeks of CMS compared with the corresponding control group (n = 20 per group). These cytokines were measured by ELISA according to the manufacturer's instructions. Data are mean ± SEM. *P < .05, **P < .01, ***P < .01 versus respective control value.
