Figure 6. Delg does not mediate the nutrient-specific control of respiration activity.
All animals were taken as mid-third instar larvae: control normal food: 4 days AED, control low yeast: 8 days AED, delg normal food 6 days AED and delg low yeast 10 days AED. Larvae were grown in normal or low yeast food as in Fig. 1. (A) qPCR for RFeSP and Blw mRNA levels, normalized to Tubulin levels. (B) Oxygen consumption assays on digitonin-permeabilized dissected fat bodies as done in Fig. 1D. ‘−’ indicates normal food, ‘+’ indicates low yeast food. In all cases, significance is indicated compared to normal fed control larvae, except in A, where in addition normal vs. low-yeast fed delg mutants are compared.