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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Free Radic Res. 2009 Jan;43(1):28–36. doi: 10.1080/10715760802534812

Figure 1. In-gel assay for peptidase activity of the proteasome.

Figure 1

Liver lysates from 3 month-old rats were analyzed for proteasome activity presence in the crude homogenate (1), >100 kDa retentate (2) and filtrate (3). Equal concentrations of the samples were separated on a 4% native PAGE gel and assayed for the presence of 26S (ATP stimulated) and 20S (SDS stimulated) proteasome complexes via visualization of ChT-L activity of the proteasome. These data show an enrichment of proteasome complexes in the retentate relative to the homogenate or filtrate.