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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2004 Sep;45(9):2906–2914. doi: 10.1167/iovs.03-1355

Figure 6.

Figure 6

Steady state levels of 4F2hc mRNA and xCT mRNA in control mice and in Tat-transgenic mice. (A) The expression of the transgene Tat in Tat-transgenic mice was demonstrated by the detection of the Tat protein (Western blot) in macrophages isolated from the Tat-transgenic mice (Tat MΦ). Macrophages isolated from control mice (Con MΦ) were negative for the transgene protein product. (B) The expression of Tat mRNA was analyzed by RT-PCR using total RNA isolated from whole eyes (Con Eye, eyes from control mice; Tat Eye, eyes from Tat-transgenic mice). 18S RNA was analyzed in the same RNA samples as internal control. (C) Semiquantitative RT-PCR for the analysis of steady state levels of mRNAs for 4F2hc, xCT, and heavy and light subunits of γ-glutamylcysteine synthetase (γ-GCS-HS and γ-GCSLS) in control RPE-eyecups (C) and in transgenic RPE-eyecups (T). 18S RNA was analyzed in the same RNA samples as the internal control.