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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2008 Aug 18;70(1):236–257. doi: 10.1111/j.1365-2958.2008.06411.x

Figure 2.

Figure 2

CsrA dependent regulation of ycdT and ydeH expression measured by Real-Time RT-PCR. (A) Induction of pBADcsrA with 0.1 % arabinose (at 0 min) leads to a rapid decrease in ycdT (squares) and ydeH (circles) transcript levels during late (OD600 1.5) exponential growth. (B) Similar results were observed during early exponential growth (OD600 0.5). (C) Induction of pBAD28 had no effect on ycdT and ydeH mRNA levels. (D) In the csrA∷kan mutant TRMG1655 (open symbols), ycdT mRNA levels (dashed line) were strongly increased compared to the wild type MG1655 (filled symbols) over the entire growth cycle. (E) Likewise, ydeH expression (dashed line) was significantly higher in the csrA mutant. (F) Analysis of ycdT, ydeH and csrA (diamonds) expression over time in the wild type indicates that ycdT and ydeH are inversely regulated with respect to csrA.