Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Description or genotype | Reference | ||
|---|---|---|---|---|
| Strains | ||||
| Escherichia coli | ||||
| MG1655 | F- λ- | Michael Cashel | ||
| BW25141 | lacIq rrnBT14 lacZWJ16 phoBR580 hsdR514 araBAD AH33 rhaBADLD78 galU95 endABT333 uidA (MluI)∷pir+ recA1 | (Datsenko and Wanner, 2000) | ||
| TRMG | MG1655 csrA∷kan | (Romeo et al., 1993) | ||
| KSB837 | CF7789 Δ(λatt-lom)∷bla φ (csrB-lacZ)1 (Hyb) ampr | (Suzuki et al., 2002) | ||
| KJ157 | KSB837 csrA∷kanr | This study | ||
| KJ227 | MG1655 csrB∷catr | This study | ||
| TWMG1655 | MG1655 csrC∷tetr | (Weilbacher et al., 2003) | ||
| KJ230 | MG1655 ΔcsrB csrC∷tetr | This study | ||
| KJ27 | KSB837 yhdA∷catr | (Jonas et al., 2006) | ||
| KJ205 | MG1655 yhdA∷catr | This study | ||
| AKP199 | MG1655 barA∷kanr | (Pernestig et al., 2003) | ||
| AKP200 | MG1655 uvrY∷catr | (Pernestig et al., 2003) | ||
| XWMGΔT | MG1655 ycdT∷catr | (Wang et al., 2005) | ||
| KJ295 | MG1655 ydeH∷catr | This study | ||
| KJ311 | MG1655 ΔydeHycdT∷catr | This study | ||
| KJ331 | KJ295 csrA∷kanr | This study | ||
| KJ330 | XWMGΔT csrA∷kanr | This study | ||
| KJ369 | KJ311 csrA∷kanr | This study | ||
| Salmonella enterica serovar Typhimurium | ||||
| MAE103 | ΔcsgBA102 adrA101∷MudJ | (Romling et al., 2000) | ||
| Plasmids | ||||
| pKD46 | temperature sensitive λ red recombinase expression vector | (Datsenko and Wanner, 2000) | ||
| pKD3 | template for mutant construction, carries chloramphenicol resistance cassette | (Datsenko and Wanner, 2000) | ||
| pBAD28 | pBAD expression plasmid | (Guzman et al., 1995) | ||
| pBADcsrA | CsrA expression plasmid, csrA is controlled by the plasmid-borne PBAD promoter | This study | ||
| pBADycdT | YcdT expression plasmid, ycdT is controlled by the plasmid-borne PBAD promoter | This study | ||
| pBADydeH | YdeH expression plasmid, ydeH is controlled by the plasmid-borne PBAD promoter | This study | ||
| pBADycdT-mut | mutagenised pBADycdT (G359A, G360A) | This study | ||
| pBADydeH-mut | mutagenised pBADydeH (G206A, G207A) | This study | ||
| pCBZ1 | pGE593, Φ(csrB-lacZ) | (Gudapaty et al., 2001) | ||
| pPYCDT | LacZ reporter plasmid, containing a ycdT-lacZ transcriptional fusion | This study | ||
| pPYDEH | LacZ reporter plasmid, containing a ydeH-lacZ transcriptional fusion | This study | ||