Table 2.
Primers used in this study.
| Primer name | Primer Sequence (5′ to 3′) |
|---|---|
| Genetic approaches | |
| ydeHKOFor2 | ATGGACTGTGCCAGTTTGGTCGGTGGATTGATCATCTGGGGCCACTCGTGTAGGCTGGAGCTGCTTC |
| ydeHKORev2 | CGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCATATGAATATCCTCCTTAG |
| ydeHKOtestFor | ACAAGGAACTGTGAAAAAG |
| ydeHKOtestRev | ATCGTTGACACAGTAGCA |
| csrBKOFor | GAGTCAGACAACGAAGTGAACATCAGGATGATGACACTTCTGCGTAGGCTGGAGCTGCTTC |
| csrBKORev | AATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTATATGAATATCCTCCTTAG |
| csrBKOtestFor | GTAGGAGATCGCCAGGAAAT |
| csrBKOtestRev | CACGCAGTAACGCTTCAAGC |
| CsrAForBAD | ACCTCTAGATCTTTCAAGGAGCAAAGAATG |
| CsrARevBAD | ACCAAGCTTGATGAGACGCGGAAAGATTA |
| pBADydeHFor | ACCTCTAGAGTGAAAAAGGAGTGGCAATG |
| pBADydeHRev | ACCAAGCTTTGAATGTTAAACGGAGCTTA |
| pBADycdTFor2 | ACCGAGCTCAGATTGGTGTAGCTTTATG |
| pBADycdTRev2 | ACCTCTAGAAGGATCAAAATGCCGCTTTA |
| YcdT-Mut-For | TAGCGCGCGTCGCCGCCGAAGAGTTTGGC |
| YcdT-Mut-Rev | GCCAAACTCTTCGGCGGCGACGCGCGCTA |
| YdeH-Mut-For | GAAACGGTTTATCGCTACGCGGCCGAAGAATTTATCATTATTG |
| YdeH-Mut-Rev | CAATAATGATAAATTCTTCGGCCGCGTAGCGATAAACCGTTTC |
| PydeHFor-EcoRI | ACCGAATTCTAAATTAGCCTGATGGCCTG |
| PydeHRev-BamHI | ACCGGATCCTGCGCGCTATTCTAACGAG |
| PycdTFor-EcoRI | ACCGAATTCTATTACTCCATGTATTGCC |
| PycdTRev-BamHI | ACCGGATCCTTCTATTATTAATAGATATAAG |
| Real-Time PCR | |
| RTrrnDFor | AGTTCCAGTGTGGCTGGTCAT |
| RTrrnDRev | GCTCACCAAGGCGACGAT |
| RTcsrAFor | TGGTGAGACCCTCATGATTGG |
| RTcsrARev | CGTACCTGGTTGCCCTTTACC |
| RTcsrBFor | CAAGGATGAGCAGGGAGCAA |
| RTcsrBRev | CGCTCCCGGTTCGTTTC |
| RTpgaAFor | TCGAACGTGAACCGCAAGA |
| RTpgaARev | ATGTACATCAACCGCACGTTTT |
| RTycdTFor | ACGCCTTATTGCGTCATGATT |
| RTycdTRev | CCCCAGGTGTCGTTGACTTT |
| RTydeHFor | AATAAGGCTATCGATGCCCACTAC |
| RTydeHRev | CGCGACCACGCTGTGA |
| RTyddVFor | TGCCCAGGTTGACGATGTC |
| RTyddVRev | ACTTCCGCGACGGTATGC |
| RTdosFor | CGCCGATGGCATTTTTTT |
| RTdosRev | ATTAACACCGCACCCATCATATT |
| RTyliEFor | TCGGTGGCTTCAGATGACTCT |
| RTyliERev | GGACGATCAAAGCAATTGTATGC |
| RTyliFFor | CCTGGACGACCTGACCAAA |
| RTyliFRev | GCGCTTTTAAATCTTCGTCAAAG |
| RTyhdAFor | GCCACGCTCACCGTTTAAGA |
| RTyhdARev | GCCGGGCAAGAATTGCT |
| RTyhjKFor | AGCCGGGAACACTGATTCTG |
| RTyhjKRev | GCATGAGGGTCGTCAATACGT |
| RTyjcCFor | GGCGCTGAAGCGTTGTTAC |
| RTyjcCRev | TCTGCCGGATTCATTATTTGC |
| 5′ RACE | |
| AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG |
| UAP | CUACUACUACUAGGCCACGCGTCGACTAGTAC |
| YcdTGSP1 | CTGACGAAACAAATAAT |
| YcdTGSP2HindIII | ACCAGGCTTGCTTGTCAAACGCTCCTCAATAA |
| YcdTGSP3HindIII | ACCAGGCTTATTGCCTACGGTCATAAATGAAAT |
| RNA Gel Mobility Shift Assays | |
| ycdT-T7 | TAATACGACTCACTATAGGGAAAGGGATCTACAACCTACAGATTGGTGTAGCTTTATGGAAAAAGACTATTTGAG |
| GC ycdT-T7 | CTCAAATAGTCTTTTTCCATAAAGCTACACCAATCTGTAGGTTGTAGATCCCTTTCCCTATAGTGAGTCGTATTA |
| ydeH-T7 | TAATACGACTCACTATAGGGCACAAGGAACTGTGAAAAAGGAGTGGCAATGATCAAGAAGACAACGGAAATTG |
| GC ydeH-T7 | CAATTTCCGTTGTCTTCTTGATCATTGCCACTCCTTTTTCACAGTTCCTTGTGCCCTATAGTGAGTCGTATTA |