Fig. 4.

exc-9 is expressed in multiple epithelia and neurons. The 2.2kb exc-9 promoter was linked to gfp and microinjected into N2 worms. Strong expression was consistently found in the excretory canal cytoplasm (arrowheads) (A) and tail whip (B), anchor cell (C), uterine seam cell (ventral view) (D), distal tip cells (oblique dorsal view) (E), as well as in the ALM neurons (arrow in A) and PLN neurons (not shown). In males, a few neurons near the tail expressed the construct (F), and weaker expression was found throughout the developing larval male tail (G). Arrows in (F) indicate Rays 4-6 in newly molted male. A translational exc-9∷gfp construct was expressed throughout the cytoplasm of the canals and substantially rescued the cystic canal phenotype of exc-9(n2669) animals (H). Anterior to the left in all panels; scale bars 50 μm.