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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2009 Aug 11;8(8):2424–2431. doi: 10.1158/1535-7163.MCT-08-0938

Figure 1.

Figure 1

Assessment of human PCFT promoter activity by luciferase gene expression reporter assays. Promoter regions of PCFT designated F1–F9 were subcloned into the pGL3-Basic vector (−). Using Lipofectamine (0.75 µl/well), HeLa cells (4000 cells seeded in a 96 well plate) were transiently co-transfected 2 days post-seeding with the pGL3 constructs (200 ng) and the phRG-B SV40 plasmid (15 ng) which contains the Renilla luciferase gene. At 16–24 hrs after transfection, luciferase activities were measured using the Dual-Glo Luciferase kit (Promega) and a POLARstar OPTIMA microplate reader (BMG LABTECH). Firefly luciferase activities were normalized with Renilla luciferase. Data represent the mean ± SE from three experiments, each performed in triplicate.