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Determination of PCFT gene copy number in HeLa wild-type, R5, and R1–11 cell lines by FISH analysis. A PCFT probe (labeled green) and a chromosome 17 painted probe (labeled red) were used to analyze metaphase spreads from A. wild-type HeLa cells, B. HeLa R5 cells, and C. HeLa R1–11 cells. Both signals are indicated by arrows.
