. Author manuscript; available in PMC: 2009 Aug 31.

Published in final edited form as: Endocrinology. 2006 Feb 9;147(5):2183–2196. doi: 10.1210/en.2005-1209

TABLE 1.

Effect of dietary fat content on energy balance of male and female WT, Mc3rKO, and Mc4rKO mice

Genotype	Low-fat diet			High-fat diet			Δ (High-fat − low-fat)

	WT	Mc3rKO	Mc4rKO	WT	Mc3rKO	Mc4rKO	WT	Mc3rKO	Mc4rKO
Gain in body mass energy (kJ/d)
Male	0.5 ± 0.2^a	2.3 ± 0.7^a	6.6 ± 1.8^b	4.4 ± 0.6^a^#	11.3 ± 1.1 ^b^*	15.2 ± 1.4^c^*	3.9 ± 0.6^a	9.0 ± 0.7^b	8.6 ± 2.9^b
Female	0.4 ± 0.2^a	1.7 ± 0.4^a	10.0 ± 1.1^b	1.5 ± 0.5^a	5.1 ± 1.3^b ^#	19.3 ± 0.6^c^*	1.1 ± 5^a	3.6 ± 1.4^a^*	9.3 ± 1.4^b
Energy intake (kJ/d)
Male	58.6 ± 2.3^a	57.0 ± 2.1^a	70.6 ± 2.2^b	62.4 ± 2.8^a	63.9 ± 1.3^a^#	84.6 ± 2.4^b^*	3.8 ± 1.6^a	6.9 ± 1.9^a	14.0 ± 4.0^b
Female	54.3 ± 1.6^a	53.5 ± 2.5^a	78.0 ± 0.5^b	58.2 ± 1.8^a^*	55.1 ± 2.6^a	90.8 ± 1.7^b^*	3.9 ± 1.4^a	1.6 ± 0.9^a	12.8 ± 1.9^b
bEE_est (kJ/d)
Male	57.6 ± 2.3	53.5 ± 1.6	60.7 ± 2.1	55.7 ± 2.8^a	47.1 ± 1.1^a ^#	61.8 ± 2.0^a,b	-1.9 ± 1.1	−6.4 ± 2.5	+ 1.1 ± 3.9
Female	53.4 ± 1.5^a	50.9 ± 2.5^a	63.0 ± 1.6^b	55.8 ± 1.7^a	47.3 ± 0.9^b	62.0 ± 1.6^c	+ 2.4 ± 1.3	−3.6 ± 2.0	−0.9 ± 3.1
ME_%
Male	0.9 ± 0.3^a	4.0 ± 1.1^a	9.2 ± 2.4^b	7.1 ± 0.9^a^*	17.6 ± 1.4^b^*	17.9 ± 1.5^b^*	6.2 ± 1.1^a	13.7 ± 1.0^b	8.7 ± 3.7^a,b
Female	0.7 ± 0.4^a	3.1 ± 0.8^a	12.9 ± 1.4^b	2.5 ± 0.8^a	9.1 ± 1.9^b ^#	21.3 ± 0.7^c^*	1.7 ± 0.7^a^*	6.0 ± 2.1^a,b^*	8.4 ± 1.9^b

Data are from the mice whose growth curves are shown in Fig. 1, A–D, and represent weekly means collected during 3 wk on a low-fat and 5 wk on a high-fat diet. bEE_est represents the proportion of kilojoules consumed less the metabolic cost of fat and protein deposition and is an estimate of the kilojoules available for non-growth-related basal metabolism and physical activity. The effect of genotype and diet on each of the parameters shown was analyzed using two-way ANOVA with repeated measures (low-fat and high-fat diet). Values not sharing a superscript letter (a, b, and c) within rows and within diets are statistically different from each other at P < 0.05; for the effects of diet within sex and within genotype:

P < 0.05;

P < 0.01.

The Δ (high-fat − low-fat) data were analyzed using two-way ANOVA with sex and genotype as independent variables. Values not sharing superscripts within rows (i.e. genotype) are statistically different from each other at P < 0.05. *, Significant effect of sex (within column) at P < 0.05.