Figure 3.

Kinetics of total lung cDC accumulation in CCR2^+/+ and CCR2^−/− mice following infection with C. neoformans. CCR2^+/+ and CCR2^−/− mice were infected with C. neoformans; total lung digests (without BAL) were performed on mice at days 0 (uninfected) and 3, 7, 10, 14, 21, and 28 post-infection. (A) Kinetics of total lung leukocyte (CD45⁺) accumulation. (B) cDC (elliptical gates) were identified using the gating strategy outlined in the Methods section and in the legend to Figure 2. Representative scatter plots (x-axis, I-A^d; y-axis CD11c) of cDC in the lungs of either CCR2^+/+ mice (left panels) or CCR2^−/− mice (right panels) at day 0 (D0; top panels) or 10 (D10; bottom panels) post-IT infection with C. neoformans. (C) Frequency of lung cDC (% of CD45⁺ cells) throughout the observed time course. (D) Numbers of total lung cDC (Total leukocytes × frequency of cDC) throughout the observed time course. CCR2^+/+, black line or bar; CCR2^−/− mice, dashed gray line or bar. Data represents a mean ± SEM of 4–7 mice assayed individually per time-point in 2–3 experiments; * p<0.05 ANOVA with Dunnet's post-hoc analysis vs. Day 0 (uninfected) of mice of the same CCR2 expression profile, ‡p<0.05 by unpaired student t-test when values from CCR2^+/+ mice or CCR2^−/− mice were compared against each other at the designated time point.