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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):610–620. doi: 10.4049/jimmunol.181.1.610

Figure 5.

Figure 5

CCR2 ligands are upregulated in the lung in response to pulmonary infection with C. neoformans. RNA was prepared from lung homogenates obtained from CCR2+/+ mice and CCR2−/− mice at baseline and at days 7, 14, 21, and 28 post-IT infection with C. neoformans. A separate RNA preparation from lung homogenates of uninfected CCR2+/+ mice (n=5) was used as a reference standard. RT-PCR was performed to evaluate the expression of the three known murine ligands for CCR2 (A–C): (A) CCL2 (MCP-1), (B) CCL 5 (MCP-3), (C) CCL12 (MCP-5) and (D) CCL11 (eotaxin-1). CCR2+/+, black bar; CCR2−/− mice, gray bar. Note difference in scales between individual panels. Data represent a mean ± SEM of 4–5 mice assayed individually per time-point in 2 experiments; * p<0.05 ANOVA with Dunnet's post-hoc analysis vs. Day 0 (uninfected) of mice of the CCR2 expression profile, ‡p<0.05 by unpaired student t-test when values from CCR2+/+ mice or CCR2−/− mice were compared at the designated time point.

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